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. 2016 Feb 17;11(2):e0149265. doi: 10.1371/journal.pone.0149265

Fig 1. Effects of serial passaging on population doubling, cell proliferation and expression of SA-β-gal in myoblasts.

Fig 1

During extensive expansion, myoblasts significantly lost their proliferation capacity as represented by a hyperbolic proliferative lifespan curve (a) and decreasing percentage of BrdU incorporation (b), while the percentage of senescent cells increased, as represented by positive SA-β-gal staining (c). For (b) and (c), the data are presented as the means ± SD, n = 3. *p<0.05 compared to myoblasts at PD14 (young), #p<0.05 compared to myoblasts at PD18 (pre-senescent) with a post-hoc Dunnett T3.