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(A) Molecular weight and the purity of the hexa-histidine tagged MazEpp and MazFpp. (B) Enzymatic activity of MazEpp and MazFpp. A 533-base synthetic RNA (RNA 500–2) was incubated with these enzymes. Lane 2, a control reaction without any enzymes; Lanes 3–6, 3 pmol of MazFpp was added. For lanes 4–6, MazE-pp was also added to each lane: 0.3, 3, and 30 pmol respectively. Lane 7, 30 pmol of MazEpp was added.
