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. 2015 Dec 15;243:719–731. doi: 10.1007/s00425-015-2441-y

Table 1.

Phosphoproteins identified by PMF query

Spot no. Exp. MW/pI a The. MW/pI b Coverage % (PM)c Accession no. Protein name Possible function Score Subcellular location Phosphorylated signald
Tyr Thr Ser
1 42.7/8.4 42.7/8.1 34 (11) BAB89081 dnaJ-like protein Stress response 122 Nucleus _ nd nd
3 40.3/8.2 35.0/8.1 50 (13) AAY57588 RING-finger E3 ubiquitin ligase Signal transduction 215 Nucleus _
+		 _
+		 _
+
4 40.0/7.5 39.0/6.3 50 (14) CAA64683 osr40c1 Defense response 218 Cytoplasm + + +
5 37.5/6.2 37.9/6.7 37 (10) BAD61512 Putative auxin-induced protein Signal transduction 118 Chloroplast _
+		 _
+		 _
+
7 37.5/6.1 41.4/6.2 44 (19) CAA77237 Reversibly glycosylated polypeptide Cell wall formation, defense response 189 Cytoplasm _
+		 nd _
+
9 27.7/6.0 20.5/6.8 26 (3) AAM20041 Putative ADP-ribosylation factor Signal transduction 62 Cytoplasm _ nd nd
10 35.2/6.7 41.4/7.0 58 (6) AAD19957 Thiosulfate sulfurtransferase Metabolism 110 Chloroplast nd _ nd
11 23.2/8.5 26.2/7.8 24 (3) AAQ06276 Uncharacterized protein Unknown 67 Chloroplast nd _ nd
12 37.0/6.4 35.7/6.6 26 (4) CAD40878 NAD(P)-linked oxidoreductase Stress response 68 Cytoplasm + + _
+
13 24.9/7.2 30.1/8.1 43 (5) AAO20076 Putative phosphatidylinositol/phosphatidylcholine transfer protein Transport 72 Mitochondrion nd nd _
14 39.6/7.6 42.1/7.0 32 (14) BAD15446 Receptor protein kinase PERK1-like protein Signal transduction 134 Cytoplasm _
+		 _
+		 _
+
15 37.3/7.1 36.5/6.7 27 (10) AAX19515 Serine/threonine protein kinase Signal transduction 122 Cytoplasm + nd nd
17 27.8/7.0 26.6/8.1 51 (10) AAC96317 Heat shock protein HSP26 Stress response 156 Chloroplast + nd nd
18 27.5/7.1 31.7/7.1 78 (11) BAD68853 Hypothetical protein Unknown 145 Cytoplasm + nd nd
19 25.2/6.4 30.5/6.7 43 (16) AAP44649 Putative ABC transporter Transport 201 Chloroplast + nd nd
20 25.6/6.7 29.2/7.3 25 (4) BAD81942 Unknown protein Unknown 65 Chloroplast + nd nd
22 42.4/6.2 35.9/6.8 46 (18) BAC99738 Putative cinnamoyl-CoA reductase Lignin biosynthesis, defense response 225 Chloroplast nd + nd
23 54.5/7.1 53.0/6.3 41 (18) AAQ64632 Glutathione reductase Detoxification, defense response 246 Cytoplasm nd + nd
24 54.4/7.2 53.2/6.3 43 (11) AAU44253 Putative trehalose-6-phosphate synthase Cell wall biogenesis, defense response 170 Nucleus nd + nd
25 54.8/7.3 49.8/7.1 46 (16) CAA51931 Phosphoglycerate kinase Metabolism 228 Chloroplast nd + nd
27 52.1/8.4 48.2/8.5 24 (8) BAC79536 Putative CBL-interacting protein kinase Signal transduction 84 Chloroplast nd + nd
28 40.4/7.7 33.4/7.7 37 (6) AAW67000 Isochorismate synthase protein Defense response, SA biosynthesis 126 Chloroplast nd + nd
30 37.4/7.7 38.5/7.3 47 (18) BAC83804 r40g2 protein Metabolism 128 Cytoplasm nd + nd
31 36.0/7.5 37.6/7.1 30 (4) AAW52722 Peroxidase 8 ROS scavenging, defense response 85 Chloroplast nd + nd
32 37.4/8.1 34.7/8.4 40 (12) AAR01635 Cellular retinaldehyde-binding protein Metabolism 136 Nucleus nd + nd
33 60.8/8.2 57.5/9.0 23 (8) AAR11387 Cytochrome P450 Defense response 95 Chloroplast nd + nd
34 37.3/5.9 35.8/5.5 38 (8) BAD05829 Zinc finger (C3HC4-type RING finger) protein -like Stress response 119 Plasma membrane nd nd _
+
35 45.6/5.8 46.6/7.0 37 (10) BAD08982 Putative RNA recognition motif (RRM)-containing protein Transcription 132 Nucleus nd nd +

aExperimental relative molecular mass (kDa)/isoelectric point

bTheoretical relative molecular mass (kDa)/isoelectric point

cSequence coverage by peptide mass fingerprinting using MALDI-TOF MS. PM, number of peptides matched

dPhosphorylated signal detected with anti-phosphotyrosine antibody, anti-phosphothreonine antibody and anti-phosphoserine antibody. − represents wheat spikes treated with H2O; + represents wheat spikes treated with F. graminearum; nd represents no detection