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. 2015 Nov 13;243:605–622. doi: 10.1007/s00425-015-2428-8

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 8 — VP-SEM analysis on tobacco leaves showing crystal extrusion and chemical composition of the excreted crystals by EDS microanalysis (insets). Details of the leaf surface and trichomes of SR1 (a–c), rolB (d–f), and rolB-AtPCS1 (g–i) plants grown for 5 weeks on refreshed MS medium without Cd, As (Control, a, d, g), or exposed either to 60 μM CdSO₄ (60 Cd, b, e, h), or 50 μM Na₂HAsO₄.7H₂O plus 60 μM CdSO₄ (50 As + 60 Cd, c, f, i). Cadmium presence in the extruded crystals after exposure to Cd and Cd plus As is detailed in the insets of b, c, e, and f (arrows). The absence of Cd in the extruded crystals in the Control treatment for all genotypes, and in all treatments for rolB-AtPCS1, is shown by the insets in a, d, g, h, and i. Bars 10 µm (b, d, i), 20 µm (a, c, e, f, g, h)