Table 3.

Perturbation experiments to monitor the transcriptomic response of S. cerevisiae to the changing temperatures.

Perturbation	Experiment type	Exposure (sampling) time	Measurement technique	Reference
Heat shock or adaptation to higher temperatures
From 25 to 37°C	Shake flasks	5, 15, 30, and 60 min	cDNA microarrays	Gasch et al. (2000)
From 25 to 37°C	Shake flasks	0, 15, 30, 45, 60, and 120 min	Affymetrix YE6100 gene chips	Causton et al. (2001)
25 or 39°C	NA	20 min	Agilent Yeast Oligo Microarray	Ruiz-Roig et al. (2010)
From 28 to 41°C	Batch bioreactor	0, 10, 30, 60, 120, 240, and 360 min	Affymetrix Yeast Genome S98 Array	Mensonides et al. (2013)
From 30 to 42°C	Shake flasks	20 min	Agilent Yeast Oligo Microarray (V2)	Hughes Hallett et al. (2014)
Cold shock or adaptation to cold
From 37 to 25°C	Shake flasks	5, 15, 30, 45, 60, and 90 min	cDNA microarrays	Gasch et al. (2000)
From 30 to 10°C	NA	15, 30, 120, 240, and 480 min	cDNA microarrays	Sahara et al. (2002)
From 30 to 10°C	Shake flasks	10, 30, and 120 min; some cases 60 h	DNA microarrays	Schade et al. (2004)
From 25 to 4°C	Batch/time series (up to 48 h)	6, 12, 24, and 48 h	cDNA microarrays	Murata et al. (2006)
30 and 12°C	Chemostat at 0.03 h−1	Sampling at steady state	Affymetrix Yeast Genome S98 Array	Tai et al. (2007)
30 and 15°C	Chemostat at 0.05 h−1	Sampling at steady state	Affymetrix Yeast Genome 2.0 Array	Pizarro et al. (2008)
28 and 15°C	Chemostat at 0.028 h−1	Sampling at steady state	Yeast Array	García-Ríos et al. (2014)