Table 4.

Perturbation experiments to monitor the transcriptomic response of S. cerevisiae to salt and osmotic shock.

Perturbation	Experiment type	Exposure (sampling) time	Measurement technique	Reference
0.4 or 0.8M NaCl	NA	10 and 20 min	DNA microarrays	Posas et al. (2000)
0.5 or 0.7M NaCl or 0.95M sorbitol	NA	45 min of 0.7M NaCl, 30 min of 0.5M NaCl, or 30 min of 0.95M sorbitol	Gene filters	Rep et al. (2000)
1M NaCl	NA	0, 10, 30, and 90 min	Gene filters	Yale and Bohnert (2001)
1M sorbitol or varying concentrations of KCl (0.0625, 0.125, and 0.5M)	Shake flasks	Sorbitol at 0, 5, 10, 20, 30, 40, 60, 90, 120, and 180 min	DNA microarrays (made in-house)	O’Rourke and Herskowitz (2004)
		KCl at 5, 10, 20, and 30 min
1M NaCl	NA	0, 10, 30, 60, 180, and 300 min	DNA microarrays	Melamed et al. (2008)
1M sorbitol	NA	10 min	Oligonucleotide microarrays	Halbeisen and Gerber (2009)
0.4M NaCl	NA	0, 2, 6, and 30 min	Yeast 6.4K microarrays	Warringer et al. (2010)
0.4M NaCl	Shake flasks	20 min	Agilent G4140A microarrays	Capaldi et al. (2008)
0.7M NaCl	Shake flasks	30 min	DNA microarrays	Chasman et al. (2014)
from 30 to 42°C	Shake flasks	20 min	Agilent Yeast Oligo Microarray (V2)	Hughes Hallett et al. (2014)
0.25, 0.5, 0.75, and 1M NaCl	Shake flasks	0, 15, 30, 45, 60, and 120 min	Yeast Gene Chip ver. 2 (DNA Chip Research Inc., Japan)	Hirasawa et al. (2006)