Table 1.
Primers used in the present work for qPCR.
| Target | Primer set | Sequence | PCR conditions |
|---|---|---|---|
| Bacillus subtilis | srfAF | 5-CAAAATCGCAGCATACCACTTTGAG-3 | 56° C |
| M15-10-1 | srfAR | 5-AGCGGCACATATTGATGCGGCTC-3 | 30 cycles |
| Dietzia sp. ZQ-4 | algF | 5-GTCCACCACGAAGCAGC-3 | 56° C |
| algR | 5-CCTACAACGGCATCAAACTG-3 | 30 cycles | |
| Rhodococcus sp. M | alkF | 5-AGTGGGCGCTCGCCCCGTCGTTCTAC-3 | 56° C |
| alkR | 5-CACGAGATACGGCGCGATCGACAGAC-3 | 30 cycles | |
| Enterobacter cloacae | 895F | 5-GGCAGCGTGTCAAACTCAA-3 | 57° C |
| FY-07 | 895R | 5-TTTACCGACGGCTCACAGAT-3 | 30 cycles |
| Enterobacter cloacae | 895F | 5-GGCAGCGTGTCAAACTCAA-3 | 57° C |
| Bacteria | 27F | 5-AGAGTTTGATCCTGGCTCAG-3 | 55° Cs |
| 1541R | 5-AAGGAGGTGATCCAGCCGCA-3 | 30 cycles | |
| Bacteria | 8F | 5-AGAGTTTGATYMTGGCTC-3 | 55° C |
| 338R | 5-GCTGCCTCCCGTAGGAGT-3 | 30 cycles | |
| Hydrocarbon degrader | alkBwF | 5-AAYCANGCNCAYGARCTNGGVCAYAA-3 | 55° C |
| alkBwR | 5-GCRTGRTGRTCHGARTGNCGYTG-3 | 30 cycles |