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. 2016 Feb 17;7:10776. doi: 10.1038/ncomms10776

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(a) Averaged difference spectrum of 100 single spectra measured in the same subject as for Fig. 1e, and the input spectra of ZnPP, PPIX and blood absorption that are used for the fitting procedure. (b) Second derivatives of the tissue difference spectrum shown in a, and of the fitted components. (c) Difference spectrum and the smooth background that remains when ZnPP, PPIX and blood absorption are removed according to the obtained fit amplitudes, indicating the effectiveness and validity of the spectral fitting procedure.