Figure 2. Influence of the 3'RR palindrome on SHM.

(a) SHM in V_H regions of ΔleftPAL, ΔIRIS and wt mice. Mice were immunised orally with sheep red blood cells for 2 weeks and intraperitoneally with 10 μg of LPS for 3 days. B220⁺GL7⁺Fas⁺ cells from Peyer's patches were sorted and pooled, and extracted DNA was amplified by PCR and submitted to high-throughput sequencing to evaluate SHM. V_H SHM values were normalised to Jκ5 SHM values. Mean values from six mice for all genotypes were reported (8–12 weeks old, male and female). (b) SHM were found all along the analysed 3'J_H4 DNA segment and hot spot of mutations were evidenced in both genotypes. Same mice as in a. (c) The proportion of transitions and transversions did not significantly differ between wt, ΔleftPAL and ΔIRIS mice. Same mice as in a. (d) Percentages of sequences with 0, 1–5, 6–10 and >10 mutations. (n): number of analysed sequences. Same mice as in a.