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. 2016 Feb 17;7:10730. doi: 10.1038/ncomms10730

Figure 4. Influence of the 3'RR palindrome on GLT and CSR.

Figure 4

(a) GLT in B splenocytes of ΔleftPAL, ΔIRIS and wt mice. Cells were stimulated with LPS±IL-4, INFγ and TGFβ for 2 days. Iγ1-Cγ1, Iγ2a-Cγ2a, Iγ2b-Cγ2b, Iγ3-Cγ3 and Iα-Cα GL transcription was investigated by real-time PCR. Mean±s.e.m. of six independent experiments with one mouse (8–12 weeks old, male and female). *P<0.05 and ***P<0.0001 (Mann–Whitney U-test for significance). Values were normalised to GAPDH transcripts. (b) CSR in B splenocytes of ΔleftPAL, ΔIRIS and wt mice. Cells were stimulated with LPS±IL-4, INFγ and TGFβ for 3 days. Cells were then labelled with anti-B220-APC antibodies and anti-IgG1-, anti-IgG2a-, anti-IgG2b-, anti-IgG3- and anti-IgA-FITC antibodies. One representative experiment out of six (one mouse per experiment) is shown (8- to 12-week-old mice, male and female). (c) Mean±s.e.m. of six independent experiments of CSR with one mouse (8–12 weeks old, male and female). *P<0.05 and **P<0.001 (Mann–Whitney U-test for significance).