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. 2016 Feb 17;7:10730. doi: 10.1038/ncomms10730

Figure 5. Influence of the 3'RR palindrome on IgH transcription and activation of epigenetic marks during CSR.

Figure 5

(a) Iγ3-Cγ3 transcription in ΔleftPAL, ΔIRIS and wt mice. CD43-depleted splenocytes were cultured for 2 days with 5 μg of LPS. RNAseq experiments were performed after depletion of rRNA. Data are the mean of two independent experiments with three mice per genotype (8- to 12-week-old-male mice) (b) ChIP analysis of H3K4me3 in Iγ3-Cγ3 in ΔleftPAL, ΔIRIS and wt mice. Data are the mean±s.e.m. of four independent experiments (8- to 12-week-old mice, male and female). *P<0.05 (Mann–Whitney U-test). (c) ChIP analysis of H3K27ac in Iγ3-Cγ3 in ΔleftPAL, ΔIRIS and wt mice. Data are the mean±s.e.m. of four independent experiments (8- to 12-week-old mice, male and female). *P<0.05 (Mann–Whitney U-test). (d) Iγ2b-Cγ2b transcription (RNAseq experiments) in ΔleftPAL, ΔIRIS and wt mice. Same mice as in a. (e) ChIP analysis of H3K4me3 in Iγ2b-Cγ2b in ΔleftPAL, ΔIRIS and wt mice. Data are the mean±s.e.m. of four independent experiments (8- to 12-week-old mice, male and female). *P<0.05 (Mann–Whitney U-test). (f) ChIP analysis of H3K27ac in Iγ2b-Cγ2b in ΔleftPAL, ΔIRIS and wt mice. Data are the mean±s.e.m. of four independent experiments (8- to 12-week-old mice, male and female). *P<0.05 (Mann–Whitney U-test).