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. 2016 Feb 17;7:10730. doi: 10.1038/ncomms10730

Figure 7. Influence of the 3'RR palindrome on Ig synthesis.

Figure 7

(a) ELISA analysis of IgG1, IgG2a, IgG2b, IgG3 and IgA in supernatants of LPS±IL-4-, INFγ- and TGFβ-stimulated splenocytes of ΔleftPAL, ΔIRIS and wt mice. Data are the mean±s.e.m. of eight experiments with one mouse (8–12 weeks old, male and female). *P<0.05, **P<0.01 and **P<0.001 (Mann–Whitney U-test for significance). (b) ELISA analysis of IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA in plasma of 10 ΔleftPAL, 9 ΔIRIS mice and 18 wt mice (8 weeks old, male and female). Mean±s.e.m. *P<0.05, **P<0.01 and **P<0.001 (Mann–Whitney U-test for significance). (c) Ovalbumin-specific IgM, IgG1, IgG2a and IgG2b. Antibody levels, detected by ELISA, are expressed in arbitrary units by comparison with control plasma values. Time after immunisation is indicated in days. Each point is the mean±s.e.m. of plasma determinations from six mice for each genotype (8–12 weeks old, male and female). One representative experiment out of two is shown.