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Interaction of TRN with RyR2, HRC, and CSQ2 at increasing Ca2+ concentrations. (A) The distal TRN deletion mutant fused to GST protein was used for pull down assay and its interacting partners in the presence of increasing Ca2+ concentrations were studied. (B) Quantitative representation of the western blot results. (Mean ± SD, *P value < 0.05, n = 3, the statistical significance was calculated with respect to the maximum interaction of the respective protein using one way ANOVA).
