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. 2016 Feb 18;6:21410. doi: 10.1038/srep21410

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(a) Subconfluent cancer cells were incubated with fresh medium. After 48 h, supernatants (upper panel) and lysates (lower panel) of cultured cancer cell lines were collected. Immunoblot analysis were performed using antibodies to importin α1 or Lamin A/C (as control). (b) Free importin α1 levels in the conditioned medium of cancer cell lines (HepG2 and AGS) were determined using ELISA. Data are means ± SD from three independent experiments. *P < 0.001.