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. 2016 Feb 18;6:21491. doi: 10.1038/srep21491

Figure 2. Apoptosis in the neural tissue of the embryos of diabetic pregnancies.

Figure 2

(a–c) TUNEL assay of E10.5 embryos in the posterior midbrain. Positive signals are green. (d–f) Merged images of TUNEL and DAPI (red) staining. (a,d) Non-DM. (b,e) DM-VEH. Arrow indicates TUNEL positive cells in the dorsal region of the neural tube. (c,f) DM-QC. Scale bar = 50 μm in all images. (g) Western blot assay of cleaved caspase-3. Upper pane: Western blots; lower panel: quantification of blot band density (mean ± SD); *p < 0.05 (DM-VEH vs. Non-DM; DM-VEH vs. DM-QC; n = 4).