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. Author manuscript; available in PMC: 2016 Feb 18.
Published in final edited form as: J Mol Biol. 2014 Jul 30;427(1):106–120. doi: 10.1016/j.jmb.2014.07.026

Figure 4. The R1070W mutation in CL4 has different effects on the gating of wild-type and ΔF508 CFTR channels.

Figure 4

(a). Western blot indicating enhancement of maturation of mutant R1070W and ΔF508 CFTRs by combined NBD1 stabilizing mutants (combo). (b and c). Single channel recordings of wild type (b) and ΔF508 CFTR (c) containing the R1070W mutant at 25 °C and 35°C. All point histograms used to calculate single channel parameters are shown to the left of each tracing. Time scale bar of 10 s is shown below the last tracing. Single channel currents (gamma, γ) and open probabilities (Po) are shown above each tracing. (d). Wild-type and ΔF508/R1070W CFTR single-channel recordings during continuous temperature ramp of 1 °C/min from 30 to 35 °C, maintained at 30 °C for 3 min before initiating the ramp and held at 35 °C for 2 min. The moment of ΔF508/R1070W functional inactivation is shown by an arrow above the tracing and appeared after about one minute at 35 °C. X-scale bar below trace is 60 s and Y-scale bar is 1 pA.