Fig. 1. NMT affinity profile for 2,3-substituted benzo[b]thiophene 1: aEnzyme apparent K i values are calculated from the IC50 values using the Cheng–Prusoff equation (see ESI†) to enable cross-comparisons. IC50 values were the mean of two or more independent determinations. Standard deviation is within 20% of reported IC50. bHsNMT affinities reported in this work refer to HsNMT1; no significant difference in inhibition was observed between HsNMT1 and HsNMT2 isoforms.18 .
