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. 2016 Feb 18;6:21710. doi: 10.1038/srep21710

Figure 3. Up-regulation of BIRC3 in response to irradiation requires the PI3K pathway.

Figure 3

(a) U87 cells were cultured in T25 flasks, pretreated with Ly294002 for 1 hour before X-ray exposure and were then irradiated. Total RNA was extracted and cDNA was synthesized in 24 hours. BIRC3 gene expression was analyzed by RT-PCR. (n = 3 independent experiments, p < 0.01). (b) U87 cells were pretreated with Ly294002 for 1 hour before X-ray exposure and then irradiated. BIRC3, phosphor-Akt and total Akt were analyzed by Western-blot 24 hours after X-ray irradiation. Data are representative of three independent experiments. p < 0.05. (c–f) U87 cells were pretreated with PI3K isoform inhibitors for 1 hour and then irradiated at 8 Gy. Cells were harvested 24 hours later and total RNA was extracted and cDNA was synthesized. BIRC3 gene expression was analyzed by RT-PCR. Similar results were obtained from three independent experiments. (p < 0.01 or no significant difference).