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. 2016 Feb 18;6:21710. doi: 10.1038/srep21710

Figure 4. Up-regulation of BIRC3 in response to RT is dependent on the STAT3 Pathway.

Figure 4

(a) U87 cells were cultured in T25 flasks and pretreated with the Stat3 inhibitor, S3I-1757 (1 hour earlier), transfected with Stat3 siRNA or control siRNA (2 days earlier) before X-ray irradiation. Total RNA was extracted and cDNA was synthesized in 24 hours. BIRC3 expression was analyzed by RT-PCR. (n = 3 independent experiments, p < 0.05). (b) U87 cells were treated as the same condition of experiment (a) and total protein was extracted 24 hours after irradiation. BIRC3 and p-Stat3 were analyzed by Western-blotting. Similar results were obtained from three independent experiments. p < 0.05.