Figure 6. BIRC3 expression (n = 3 independent experiments, p < 0.05) treatment induced apoptosis in vivo.

Mouse GBM xenografts were set up by injecting 2 × 10⁶ U251 control or U251 BIRC3 cells on the right and left flank, respectively, of the same mice. Two weeks later, mice with similar tumor size on both sides were treated with either 50 mg/kg of TMZ orally or vehicle control daily for 5 days. Mice were injected with 50 μL NIR-FLIVO747 via the lateral tail vein after the 5th dose of TMZ. 4 hours later, mice were imaged using the PerkinElmer FMT2500 to compare tumor cell apoptosis on both sides. U251 control cells were injected in the right flank while U251 BIRC3 cells were injected in the left flank. (a) Vehicle treatment. (b) TMZ treatment. Data are representative of 5 mice. (c) Average fluorochrome concentration in the tumor area on both sides (p < 0.05; n = 5).