Figure 7. Decreased apoptosis in TMZ treated BIRC3 over-expressing xenografts.

Mouse GBM xenografts were set up by injecting 2 × 10⁶ U251 control or U251 BIRC3 cells on the right and left flank, respectively, of the same mice. Two weeks later, mice with similar tumor size on both sides were treated with either 50 mg/kg of TMZ orally or vehicle control daily for 5 days. Mice were then sacrificed and tumor tissue on both sides were isolated, cut and fixed in 10% neutral formalin. Cleaved caspase3 immunohistochemistry was performed as described in the Material and Methods section. (a,c) U251 control. (b,d) U251 BIRC3. 5 mice were included in this study and similar results were observed in each animal.