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. 2016 Feb 18;6:20156. doi: 10.1038/srep20156

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(A) The expression of RORγt mRNA in lung and spleen tissue at different time points. The error bars indicate SEM. The different superscript letters (a–c) represent a significant difference of these columns (p < 0.05). (B,C) Western blot analysis tested the expression of RORγt protein in the lungs and spleen, respectively. (D) Histopathological and immunohistochemistry findings (HE: ×200; IHC: ×400). The tan color indicates RORγt-positive staining. Arrows indicate positive cells. All mice were wild type, with 4~6 mice per group.