Figure 5. Insufficient developmental dendrite spine and synapse loss in suppressed mice hippocampus.

(A) CA1 pyramidal neuron cell body layer, myelinated axons are shown in control and PH-Tau_low mice (arrow head), but absent in PH-Tau_high mice. (B) Synapses in CA1 stratum radiatum area, lower panel is the magnified images of square boxes, decreased post-synaptic density and enlarged pre-synaptic portion are shown in PH-Tau_low mice. (C) Quantitation of the number of synapses in the CA1 stratum radiatum area, significant loss of synapses in PH-Tau_low mice is observed. Decrease in the length of the post-synaptic density was observed in both PH-Tau_low and PH-Tau_high mice. (D) Representative Western blot of mouse hippocampus homogenate. The levels of synaptophysin, PSD95, and β-III-tubulin were measured by densitometry and normalized with the levels of GAPDH (*P < 0.05, **P < 0.01, ***P < 0.001).