Figure 1. Expression of ENPP6.

(A) Expression of ENPP6 protein in adult mice tissue. Total protein (20 μg/lane) from various mouse tissues was examined by Western blot analysis. Note that the molecular weight of ENPP6 detected in each tissue varies, possible due to the difference in glycosylation. (B) Immunohistochemical analysis of ENPP6 and MBP in central and peripheral nerve system in adult mice. Magnified view of the brain shows that ENPP6 was predominantly detected in the myelin sheath but not in the cell bodies of oligodendrocytes or other neural cells. (C) Expression of ENPP6 and myelin-associated proteins (MAG and MBP) in developing mouse brain. ENPP6, MAG and MBP were detected from P4, P8 and P12, respectively. (D) Immunohistochemical analysis of ENPP6 in developing mouse brain. ENPP6 expression was evaluated in developing mice brains from P2, P4, P12 and P14. The nucleus of each cell was visualized by hematoxylin staining. Note that ENPP6 is localized around the nucleus at P2 and P4 (arrows). In contrast, ENPP6 is strongly expressed in the myelin sheath but rarely in the cell bodies or perinuclear region at P12 and P14. (E) Expression of ENPP6 protein during the differentiation of OPCs to oligodendrocytes. 24, 48, 72 and 96 h after thyroid treatment, ENPP6 expression was examined. (F–H) Immunofluorescence analysis of ENPP6 during oligodendrocyte differentiation. (F) ENPP6 (red) and the OPC-specific marker, NG2 (green). (G) ENPP6 (green) and MAG (red). Notably, MAG was not expressed in the cell bodies expressing ENPP6 at 48 h after hormonal treatment (arrows), whereas both ENPP6 and MAG were expressed at 72 h (arrows). (H) ENPP6 (green) and the astrocyte marker, GFAP (red). Astrocytes do not express ENPP6. (I) Immunohistochemical analysis of ENPP6 in adult mouse liver. A magnified view was also shown. (J) Immunofluorescence analysis of ENPP6 (red) and CD146 (green) in the liver.