Figure 2. Choline deficiency-like phenotypes of ENPP6 KO mice.

(A) ENPP6 KO mice are prone to develop fatty liver. Liver section from wild-type and ENPP6 KO mice were stained with oil red O. Mice were also fed with choline-deficient diet and the liver section was examined. (B,C) ENPP6 KO mice show abnormal development of myelin. (B) Kluver-Barrera’s staining of brain sections from adult wild-type and ENPP6 KO mice (left four panels). Electron micrographs of myelin (right four panels). Magnified views are shown in the lower panels. Layers of myelin sheath are indicated by arrows. (C) Kluver-Barrera’s staining of brain sections from P14 wild-type and ENPP6 KO mice (left panels). Electron micrographs of myelin (center panels). Immunofluorescence analysis of MAG in myelin (right panels). Note that myelin fibers and sheath are less developed and that immature oligodendrocytes with round shapes are evident in ENPP6 KO mice.