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. 2016 Feb 18;6:21747. doi: 10.1038/srep21747

Figure 1. 42 °C is the critical temperature for feeder-less human iPS cells.

Figure 1

(a) Human iPS cells cultured on laminin E8 fragment were cultured at 37 °C, 40 °C, 41 °C, or 42 °C for 1 or 2 days. Upper panels are representative of phase-contrast images. Bars, 100 μm. Middle panels, Hoechst staining images. Bars, 200 μm. Lower panels, montage images of 36 fields (6 × 6) of Hoechst staining images (original magnification of each field is ×20). Multiple images from the same sample were acquired using the same microscope settings. (b) The cell number at each condition was calculated and shown in the graph (n = 3). *p < 0.01 vs. day 0. (c) Representative images of TUNEL staining (green). Nuclei were stained with Hoechst. (d) Percentage of TUNEL(+) cells was calculated and shown in the graph (n = 3). *p < 0.01 vs. day 0.