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. 2016 Feb 18;6:21747. doi: 10.1038/srep21747

Figure 5. Elimination of remaining iPS cells in cardiac cell sheet preparation.

Figure 5

(a) The mRNA expression in cardiac cells after cardiac differentiation of human iPS cells from each time point at 42 °C (n = 3). Y-axis indicates relative gene expression compared with GAPDH. *p < 0.05 vs. pre. (b) The mRNA expression of Lin28 and Oct4 of cardiac cells after cardiac differentiation of human iPS cells from each time point at 42 °C (n = 3). Y-axis indicates relative gene expression compared with GAPDH. *p < 0.05 vs. pre. (c) The relative mRNA expression of Lin28 and Oct4 of iPS cell-derived cardiac cells at 48 hours in 42 °C (n = 3, iPS cell = 100). Y-axis indicates relative gene expression compared with undifferentiated iPS cells cultured on MEF. (d) Effects of 42 °C culture on cell sheet fabrication. Four days after starting culture of iPS-derived cardiac cells in temperature-responsive multi-well culture plates at 37 °C, monolayered cell sheets were fabricated following lowering of culture temperature (upper). Even if cells were cultured at 42 °C from day 1 to day 4 (middle), or from day 1 to day 3 (lower), cell sheets were fabricated following lowering of culture temperature at day 4. Left, culture scheme. Right, macroscopic view of monolayered cell sheets from the different experiments.