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. 2016 Feb 18;6:19943. doi: 10.1038/srep19943

Figure 2. GRHL2 is required for the epithelial phenotype of EOC and the distribution of E-cadherin at cell–cell junctions.

Figure 2

(a) The morphologies of EOC cell lines (PEO1, OVCA420, OVCA429) stably infected with control shRNAs (shLuc, shNon) and two different GRHL2-targeting shRNAs (shGRHL2 #10, shGRHL2 #12). Scale = 100 μm. (b) Western blots of GRHL2, E-cadherin, vimentin and β-actin in EOC stable cell lines. Upper bands in the GRHL2 panel were deemed non-specific. Full-length blots are presented in Supplementary Fig. S10. (c) Immunofluorescence stainings of E-cadherin (red) and β-catenin (green) in the EOC stable cell lines. Nuclei were stained blue (DAPI). Scale = 50 μm. (d) Scatter plots showing molecular subtype enrichment scores for OVCA429 shNon (n = 2) and shGRHL2 cells (shGRHL2 #10, n = 2 and shGRHL2 #12, n = 3) using single-sample Gene Set Enrichment Analysis (ssGSEA), based on microarray gene expression. Bars indicate mean values ± SEM. Unpaired t-tests were used. (e) EMT enrichment scores of OVCA429 shGRHL2 cells were higher as compared with that of shNon cells, based on unpaired t-tests. Bars indicate mean values ± SEM.