BPA increases and accelerates adipogenic gene induction in ASCs. (A) Induction of the early adipogenic gene marker DLK was measured in pooled ASCs (n=3) treated with 1 μM BPA or DMSO vehicle in fat differentiation media (FDM) treated for 7, 14, or 21 days. (B) Mid adipogenic induction markers analyzed included CEBPA. (C) Late adipogenic induction markers analyzed included IGF1, PPARγ, and LPL. (D) Western blot analysis for LPL protein levels in ASCs at baseline or following 7 days of treatment with either DMSO vehicle or 1 μM BPA in FDM. Bars, ±s.d. ****P<0.0001 and **P<0.01.