Figure 3.
Density-dependent glutamine metabolism. (A): Fold-change in cell number as a function of plating density in the presence or absence of glutamine in the medium for 48 hours; (B): CFU-F efficiency as a function of plating density in the presence or absence of glutamine in the medium for 48 hours; (C): ATP content as a function of plating density in the presence or absence of glutamine in the medium for 48 hours; (D): Growth inhibition as a function of plating density in the presence of 100 μM aminooxyacetate for 48 hours. Data represent the mean of at least three independent determinations; errors represent the standard error in the mean. Δ: percentage difference of ATP between control and additional treatment divided by control. *, p < 0.05; **, p < 0.01. Abbreviations: AOA, aminooxyacetate; CFE, CFU-F efficiency; HD, high density; LD, low density.