Table 2.
PCR primers used in this study for the amplification of the mitochondrial NADH dehydrogenase 1 (ND1) and cytochrome c oxidase subunit 1 (COI) genes, as well as the control region (CR) from aDNA extracts from bones of Atlantic walrus (Odobenus rosmarus) from Svalbard
| Amplicon | Primer | Forward primer sequence (5′–3′) | Primer | Reverse primer sequence (5′–3′) |
|---|---|---|---|---|
| ND1-1 | ND1-1Fa | ACCCCGCCTGTTTACCAAAAACAT | ND1-1R | TATATTCCCGCCTCTTCACG |
| ND1-2 | ND1-2F | GCCACACGAGGGTTCTACTG | ND1-2R | TCGGGGGTTGTGCTATACTC |
| ND1-3 | ND1-3F | GGGACAGCAATTTAGGTTGG | ND1-3Rb | TGTCCTGATCCAACATCGAG |
| ND1-4 | ND1-4Fb | GGGATAACAGCGCAATCCTA | ND1-4R | TAGTCCTAAGGCGCTTCGTC |
| ND1-5 | ND1-5F | CAAGAGAGACAAGGCCCACT | ND1-5R | GGAAGGCTACGGCTAGAAGAA |
| ND1-6 | ND1-6F | CTTTTACCCCCAGAGGTTCA | ND1-6Rb | TGGTCGTAAAGGTTCCTTGG |
| ND1-7 | ND1-7Fb | GGACCATACGGACTTCTCCA | ND1-7R | CGGCTAGGCTTGATATTGCT |
| ND1-8 | ND1-8F | CCTATACCGTACCCTCTCATCA | ND1-8R | TGGTTAAAGAGAATGATCCGTTT |
| ND1-9 | ND1-9F | GCCATCATTCTCTTGTCCGTA | ND1-9Rb | CCAGGAAGAATAGGGCGAAT |
| ND1-10 | ND1-10Fb | CAGAATTAGTATCAGGCTTCAACG | ND1-10R | ATGATGCCCGAATTCATAGG |
| ND1-11 | ND1-11F | TTCACAACCACTCTATTTCTTGGA | ND1-11R | TTGTGGAGGAACACTTGCTG |
| ND1-12 | ND1-12F | TGCATATGACACATGGCTCTG | ND1-12Rb | GGTATGGGCCCGATAGCTTA |
| COI-1 | COI-1Fb | ACAAGGACATCGGCACTCTC | COI-1Rb | GCTCCGATTATTAGGGGAACT |
| COI-2 | COI-2Fb | GCCCATGCATTCGTAATAATC | COI-2Rb | GATGGTCCTGCGTGAGCTA |
| COI-3 | COI-3Fb | GAACCGGATGAACCGTCTAC | COI-3Rb | CCGCTGTAATTAATACGGACCA |
| COI-4 | COI-4Fb | CTCCCGCAATATCCCAATAC | COI-4Rb | TTCCGAATCCTGGTAGAATGA |
| CR-1 | CR-1Fb | GCCTATTGCCGGTATAATCG | DL-1Rb | TGTGATGGTACAGTAGGGGTGA |
| CR-2 | CR-2Fb | CTGACGCCCTACCATTCATA | DL-2Rb | AAGGGTTGCTGGTTTCTCG |
| CR-3 | CR-3Fb | AATTCACTTGGTCCGTCAAGC | DL-3Rb | TTATGTGTGATCATGGGCTGA |
| CR-4 | CR-4Fb | TGGGACATCTCGATGGACTT | DL-4Rb | CGTGTATGTCCTGTGACCATT |