Table 2.
Key process parameters of anaerobic glucose conversion of P. putida F1 in the anode compartment of a BES using [Co(bpy)3]3+/2+ or [Fe(CN)6]3−/4− as electron acceptors with the anode potential poised at +0.697 V vs SHE
| [Co(bpy)3]3+/2+ | [Fe(CN)6]3−/4− | |
|---|---|---|
| Carbon balance (%) | 99.6 | 97.6 |
| Coulombic efficiency (%) | 98.5 | 93.3 |
| Yields [molproduct/molglucose] | ||
| Y 2KGA | 0.90 ± 0.03 | 0.90 ± 0.02 |
| Y acetic acid | 0.07 ± 0.01 | 0.14 ± 0.01 |
| Y gluconic acid | 0.31 ± 0.06 | 0.09 ± 0.03 |
| 0.25 ± 0.03 | 0.09 ± 0.04 | |
| Y electrons | 3.94 ± 0.11 | 3.88 ± 0.07 |
| Rates [mmol/(gCDW h)] | ||
| r glucose | −0.81 ± 0.14 | −1.10 ± 0.21 |
| r acetic acid | 0.06 ± 0.01 | 0.16 ± 0.03 |
| r 2KGA | 0.73 ± 0.13 | 0.99 ± 0.19 |
| r gluconic acid | 0.25 ± 0.06 | 0.10 ± 0.04 |
| −0.20 ± 0.04 | −0.10 ± 0.05 | |
| r electrons | 3.18 ± 0.55 | 4.28 ± 0.82 |
Data are fitted with linear regression using datasets from ten ([Fe(CN)6]3−/4−) and four ([Co(bpy)3]3+/2+) biological replicates with a total of 79 and 36 samples, respectively (compare Additional file 1: Fig. S3). Carbon balance is calculated from the fitted rates considering carbon content of molecules and assuming equimolar CO2 production when making acetate from glucose. Gluconic acid is a product in the first 100 h and serves as a substrate thereafter; hence, 2 yields and rates are given