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. 2016 Feb 18;6:21228. doi: 10.1038/srep21228

Figure 4. miR-182 downregulates Bcat2, Foxo3 and Adcy6 in PlGF mice and MEFs.

Figure 4

(a) Alignment of mmu-miR-182 with putative 3′UTR target sites: one site in Bcat2 and Foxo3 and 4 sites in Adcy6. miR-182 seed sequence and the corresponding target sites are indicated in green box. Complementary bases are shown in red color. (b) Bcat2, Foxo3 and Adcy6 transcripts are downregulated in PlGF mice and control miR-scramble-treated PlGF mice, compared with controls. Anti-miR-182 treatment completely restored Bcat2 and Foxo3 expression and partially restored Adcy6 expression in PlGF mice. n = 4 (controls); 4 (PlGF); 4 (PlGF + control miR-scramble); 5 (PlGF + anti-miR-182). (c) Reduction of Bcat2, Foxo3 and Adcy6 expression in MEFs transfected with miR-182 mimic (50 nM). n = 4 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.