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. 2016 Feb 18;6:21144. doi: 10.1038/srep21144

Figure 4. Rhus coriaria inhibits the migration of MDA-MB-231 cells across monolayer of TNF-α activated HUVECs and reduces their adhesion to HUVECs and lung endothelial cells (HMVEC-L).

Figure 4

(A) Inhibition of transendothelial migration of MDA-MB-231 through TNF-α stimulated HUVECs. Confluent monolayers of HUVECs were pre-incubated with TNF-α (25 ng/mL) for 6 h, and transendothelial migration was evaluated after overnight incubation at 37 °C. Values represent means ± SEM, n = 3. Statistical analysis was performed using one-way ANOVA (*p < 0.05, **p < 0.005). (B,C) Inhibition of adhesion of MDA-MB-231 cells to HUVECs (B) and HMVEC-L (C). MDA-MB-231 cells were treated overnight with vehicle (0.2% ethanol) or indicated concentrations of RCE then seeded on top of the confluent endothelial cells and allowed to adhere for 60 min as described in Materials and Methods. Adhesion of MDA-MB-231 cell was represented as percentage of cells that have adhered to HUVECs (B) or HMVEC-L (C). Data represent means ± SEM of three independent experiments. Statistical analysis was performed using one-way ANOVA (*p < 0.05, **p < 0.005).