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. 2016 Feb 18;6:21197. doi: 10.1038/srep21197

Figure 2. HAS2 is a candidate target gene of miR-26b.

Figure 2

(A) HAS2 expression level in healthy follicles and atresia follicles. H: healthy follicles; A: atresia follicles. mRNA and protein levels were determined by RT-qPCR and western blot analyses, respectively. Cropped gels were used for our western blot results. (B) miRNAs that target the HAS2 gene were predicted by five algorithms: TargetScan, miRanda, PITA, DINA-mT, and RNAhybrid. Twenty-four miRNAs were commonly predicted. (C) Five miRNAs predicted to regulate HAS2 were differentially expressed during follicular atresia in porcine ovaries. H: healthy follicles; EA, early atresia follicular. PA, progressively atretic follicles. (D) The miR-26b-binding site within the 3′-UTR of HAS2 was predicted by RNAhybrid. (E) Alignments of miR-26b mature sequences and the 3′-UTR of HAS2 from pig and other mammals. Red letters indicate the seed sequences of miR-26b. Asterisks indicate complementarity. *p < 0.05, **p < 0.01.