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. 2016 Feb 18;6:21117. doi: 10.1038/srep21117

Figure 6. Characterization of a mammary tumor cell line, “SUM149PT-TetOn-TuD-141/200c” which harbours the Tet-inducible TuD-141/200c expression system.

Figure 6

(a) Schema for the treatment and ESA expression pattern of SUM149PT-TetOn-TuD-141/200c cells. SUM149PT cells were transduced with pXL001 and subsequently with either pLSB-Empty or pLSB-Tete7SK-TuD-141/200c and selected (cultures A,B). The pLSB-Empty transduced cells were cultured in the absence of Dox. Culture B was grown in the presence of Dox for 32 days and the cellular fraction with very low ESA levels was then sorted. One part of this fraction was subsequently cultured for 35 days in the presence of Dox (culture C), whereas the other part was cultured in the absence of Dox for 28 days from which the ESA(+) fraction was sorted and further grown in the absence of Dox for 7 days (culture D). (b) Expression levels of several mRNAs after TuD-141/200c induction and a release from its inhibiting activity. Total RNA was prepared from cultures (A–D) described in (a), and all of the mRNAs examined in Fig. 3a–d were determined by qRT-PCR. The relative expression levels are represented by the mean ± SD, taking the level of culture A as 1.0 (n = 3).