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. 2016 Feb 18;13:42. doi: 10.1186/s12974-016-0506-x

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© Quast et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — IVIG-derived F(ab’)₂ fragments do not induce cell death of murine neutrophils. Flow cytometric analysis of cell death by annexin V-FITC/PI staining (a) or DNA fragmentation (b) of cells cultured in presence of 13.3 mg/ml F(ab’)₂ or 110 ng/ml crosslinked Fas-L (positive control). Results of 15-hour cultures are shown. Data are representative of three independent experiments. Quantitative analysis is indicated as the percentage representative of each quadrant (a) or of gated subdiploid DNA (b)