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. 2016 Feb 18;15:41. doi: 10.1186/s12934-016-0435-5

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© Gao et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Hydrolysis halos formed by different clones isolated from marine mud metagenomic library. Activity was observed on 0.5 % (v/v) tributyrin containing LB agar after 48 h of incubation at 37 °C. Five clones showed the largest hydrolysis halos and were chosen for further study