Table 2.
Real time-qPCR primer and product size
| Gene | Sequence (5′ → 3′) | Size (bp) | Cycle | Annealing temp. | Ref. | |
|---|---|---|---|---|---|---|
| β-Actin | (S) | ACAGAGCCGCCTCTGCC | 124 | 45 | 58 °C | Genebank AB009345 |
| (AS) | ACAAGCCTGAGCCGTTGTC | |||||
| BSP | (S) | ATACCATCTCACACTAGTTATAATG | 116 | 45 | 58 °C | Genebank NM000493 |
| (AS) | AACAGCATAAAAGTGTTCCTATATC | |||||
| COLI | (S) | GCAAGAGAGAAAAGAGTGAACC | 103 | 45 | 58 °C | Genebank AY633663 |
| (AS) | GTGGCTCAAGCAGAACCAG | |||||
| Cbfa-1 | (S) | CAGTCACATCAGGATATCC | 117 | 45 | 58 °C | Genebank L38480 |
| (AS) | ATGCTGCTGATCTGGAAGA | |||||
| OCN | (S) | CTCCAGGCACCCATCTTTAC | 121 | 45 | 58 °C | Genebank NM00095 |