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. Author manuscript; available in PMC: 2016 Feb 18.
Published in final edited form as: Nat Protoc. 2011 Mar 24;6(4):502–508. doi: 10.1038/nprot.2011.315

Figure 3.

Figure 3

‘Double-bubble’ pattern. (a) Reference image for anatomy; slice is a thalamocortical preparation. (b) ΔF/F image after stimulation in layer 5; note the oblong activation in layers 2/3. (b,c) Gray circle in b corresponds to the stimulation site pixels analyzed in c; the black circle in b corresponds to the response site pixels analyzed in c. Color bar represents ΔF/F activation values (.043 = 4.3 percent). (c) ΔF/F traces for stimulation (gray) and response (black) sites. Stimulation parameters: 50 μA, 20 Hz, 10 pulses and 5 ms pulse width. Traces are averages of 5 × 5 pixels (277.9 μm2). Time in seconds is on the x axis and ΔF/F is on the y axis.