Fig. 5.—

KfpTAC3 binds dsDNA nonspecifically and condenses nucleoids in E. coli. (A) Coomassie Brilliant Blue-stained gel after SDS-PAGE of purified partial KfpTAC3 recombinant proteins (80 pmol). (B) Electrophoretic mobility shift assay of partial KfpTAC3 recombinant proteins. The 100-bp DNA ladder was incubated with various amounts of purified fusion protein (80, 40, 20, and 10 pmol). (C) DH5α E. coli cells with the pQE80l vector containing the sequence of the full-length KfpTAC3 gene or with the empty vector for control. KfpTAC3 overexpression was induced with 1 mM IPTG for 4 h. The bacterial nucleoids were stained with DAPI. (D) Effect of nucleoid condensation mediated by KfpTAC3 on DH5α E. coli cells. Escherichia coli cells expressing the pQE80l vector containing the KfpTAC3 gene were grown at 37 °C, and after the addition of IPTG, the OD600 was measured at different time points. Error bars represent standard deviation. There are three independent data points.