Table 1.
Summary of BLAST Results, Assembly Statistics, ORF Prediction, and Annotation of Unigenes with a Nematode Top BLAST Hit of Two Targeted Locations and Species: HMMV (deep sea, Halomonhystera hermesi) and Western Scheldt (intertidal, GD1)
| Stage | Deep Sea | Intertidal | Total | |
|---|---|---|---|---|
| BLAST result | Unigenes with at least one BLAST result | 33,079 | 42,409 | 75,429 |
| Nematode assembly | Contigs with nematode top BLAST hit (unigenes) | 5,498 | 8,588 | 14,086 |
| N50 length (bp) | 1,378 | 1,169 | ||
| Max length (bp) | 7,253 | 8,783 | ||
| Average length (bp) | 943.07 | 837.39 | ||
| Average coverage per base | 14.07 | 20.43 | ||
| GC content unigenes (%) | 56.11% | 48.71% | ||
| ORF prediction | Unigenes with predicted ORF (CDS ≥ 200bp) | 5,227 | 8,102 | 13,329 |
| KEGG annotation | Unigenes assigned to KO identifier | 2,985 | 4,548 | 7,533 |
| Unique KO identifiers | 1,648 | 1,246 | 2,894 | |
| Go annotation | Mapped to GO terms | 5,014 | 7,822 | 12,836 |
| Unigenes annotated with GO terms | 3,657 | 5,636 | 9,293 |