Fig. 3.—

CLANS clustering of SpaCBA and sortase protein sequences from the Lactobacillus genus. The color coding of the species is as follows: red—L. casei, green—L. paracasei, and navy blue—L. rhamnosus. Proteins of other species present on panels, but not colored, include L. plantarum, L. brevis, L. gasseri, L. johnsonii, L. delbrueckii, L. crispatus, and L. ruminis. Proteins encoded by L. rhamnosus GG and in pLOCK 0919 are indicated by salmon-colored larger squares. The intensity of the connecting lines reflects the level of sequence similarity. In cases of L. casei and L. paracasei, SpaB and SrtC are overlaid because of identical protein sequences. The sortase sequences do not form any well-defined groups excluding a few L. casei/L. paracasei clans.