Table I.

shRNA sequences used in the present study.

shRNA	Target location	Target sequence	shRNA synthetic sequences
eIF4E-shRNA1	2094	CCAAAGATAGTGATTGGTTAT	Sense: 5′-CACCGCCAAAGATAGTGATTGGTTATTTCAAGAGAATAACCAATCACTATCTTTGGTTTTTTG-3′ Antisense: 5′-GATCCAAAAAACCAAAGATAGTGATTGGTTATTCTCTTGAAATAACCAATCACTATCTTTGGC-3′
eIF4E-shRNA2	1849	GGAGGACGATGGCTAATTACA	Sense: 5′-CACCGGAGGACGATGGCTAATTACATTCAAGAGATGTAATTAGCCATCGTCCTCCTTTTTTG-3′ Antisense: 5′-GATCCAAAAAAGGAGGACGATGGCTAATTACATCTCTTGAATGTAATTAGCCATCGTCCTCC-3′
eIF4E-shRNA3	1970	GTGGCGCTGTTGTTAATGTTA	Sense: 5′-CACCGTGGCGCTGTTGTTAATGTTATTCAAGAGATAACATTAACAACAGCGCCACTTTTTTG-3′ Antisense: 5′-GATCCAAAAAAGTGGCGCTGTTGTTAATGTTATCTCTTGAATAACATTAACAACAGCGCCAC-3′
NC-shRNA			Sense: 5′-CACCGTTCTCCGAACGTGTCACGTCAAGAGATTACGTGACACGTTCGG AGA ATTTTTTG-3′ Antisense: 5′-GATCCAAAAAATTCTCCGAACGTGTCACGTAATCTCTTGACGTGACACGTTCGGAGAAC-3′

shRNA, short hairpin RNA; eIF4E, eukaryotic initiation of transcription factor 4E; NC, negative control.