Table II.

mRNA and protein expression levels of eIF4E in transfected cells.

Treatment	Relative expression levels
	mRNA	Protein
Mock	0.918±0.150	0.857±0.069
NC	0.905±0.068	0.829±0.102
eIF4E-shRNA1	0.541±0.092ab	0.495±0.093ab
eIF4E-shRNA2	0.428±0.137ab	0.339±0.080ab
eIF4E-shRNA3	0.361±0.083ab	0.254±0.086ab

mRNA expression was assessed by reverse transcription-quantitative polymerase chain reaction and normalized against β-actin. The protein expression was assessed by western blotting and normalized against GAPDH.

^aP<0.01, vs. the mock shRNA;

^bP<0.01, vs. the NC shRNA. shRNA, short hairpin RNA; eIF4E, eukaryotic initiation of transcription factor 4E; NC, negative control.