Fig. 1.
a A492 value for the eluted fraction by different elution buffer in s-ELISA. A (4M MgCl2 with 75 mM HEPES, pH6.5); B (3M KSCN); C (100 mM Glycine HCl, pH 3.0); P positive control; N negative control. b SDS-PAGE analysis of proteins of BTV-1 purified by immuno-affinity chromatography. Viral proteins were resolved on 10 % polyacrylamide gel and stained with silver nitrate. Lane-1 Protein molecular weight marker (#PG500-0500PI, Puregene); Lane-2 BTV-1 infected BHK-21 cell culture lysate showing mainly the cellular proteins; Lane-3 Major structural proteins (VP1, VP2/VP3, VP5, VP7) of BTV-1 purified by immuno-affinity chromatography method