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. Author manuscript; available in PMC: 2016 Feb 18.
Published in final edited form as: Procedia Vaccinol. 2015;9:16–23. doi: 10.1016/j.provac.2015.05.003

Figure 6.

Figure 6

The upper map (pARK-C series) displays the KpnI-BamHI region of the pARK-C series plasmids, with the cspD2 promoter labelled Pcs, His-tag shown as red box, and gvpC gene (with C1, C2, C3, and C4 regions marked as in Figure 2) shown as blue arrow. The four lower maps (labelled pDRK-C1-L to C4-L) show the KpnI-BamHI regions of pDRK-C-L plasmid series containing the gvpA promoter (labelled Pgv), His-tag (red box), C1, C2, C3, and C4 regions of gvpC (blue boxes), and codon optimized Gaussia princeps luciferase gene (yellow arrow). The corresponding sites of KpnI, NdeI, and BamHI cleavage are indicated, while the AfeI sites at the GvpC-luciferase gene boundaries are not shown20.