1a |
Literature searches; electronic resources and tools (e.g. Pymol) |
Problem set |
1b |
Basics of pipetting with the micropipette |
Practical (wet‐laboratory) |
2 |
Critically reading the primary literature (in‐laboratory discussion) |
Problem set |
3 |
Making biochemical buffers and solutions |
Calculations |
4 |
Enzyme kinetics (using LDH as a test system) |
Laboratory report |
5 |
Analyzing enzyme kinetics data (computer laboratory) |
6 |
Computational biology, I: Bioinformatic tools, web/database resources |
Problem set |
7a |
General molecular cloning and transformation |
Laboratory report |
7b |
Recombinant protein expression and SDS‐PAGE |
8 |
Protein purification, I: Gel‐filtration and ion‐exchange chromatography |
9 |
Protein purification, II: Affinity chromatography |
10 |
Quantitative protein concentration determination; ligand‐binding |
Laboratory report |
11 |
Computational biology, II: Molecular visualization, modeling, docking |
POI report |