1 |
Organize reagents and buffers and finalize protocol for assay |
— |
2–3 |
Express, purify, and quantify POI |
Revised POI report; chemical order request (reagent inventory) |
4–5 |
Find workable solution/buffer conditions, optimize protein concentration for enzyme kinetics assays, establish controls for these assays |
— |
6 |
Group meeting preparation; evaluation of assay; begin determining kinetics parameters (K
M, k
cat, etc.) |
Draft of Materials & Methods section of final POI report |
7–8 |
Experimental determination of kinetic parameters for each POI |
GM presentation 1 |
9 |
Present GM 1; begin systematic variation/perturbations of kinetics assays (e.g. substrate variation) |
Drafts of Introduction, figures, and Future Directions sections |
10–12 |
Repeat assays for statistical replicates or troubleshooting; final refinements and kinetics data‐collection |
GM presentation 2; poster presentation; final POI report (manuscript format) |