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. 2015 Jul 6;43(4):245–262. doi: 10.1002/bmb.20873

Table 2.

Second‐semester timeline

Week Module Assignment type
1 Organize reagents and buffers and finalize protocol for assay
2–3 Express, purify, and quantify POI Revised POI report; chemical order request (reagent inventory)
4–5 Find workable solution/buffer conditions, optimize protein concentration for enzyme kinetics assays, establish controls for these assays
6 Group meeting preparation; evaluation of assay; begin determining kinetics parameters (K M, k cat, etc.) Draft of Materials & Methods section of final POI report
7–8 Experimental determination of kinetic parameters for each POI GM presentation 1
9 Present GM 1; begin systematic variation/perturbations of kinetics assays (e.g. substrate variation) Drafts of Introduction, figures, and Future Directions sections
10–12 Repeat assays for statistical replicates or troubleshooting; final refinements and kinetics data‐collection GM presentation 2; poster presentation; final POI report (manuscript format)