Table 1.
Binding of 4E5A and Variants to EDIII of DENV-1 to −4 Determined by Competition ELISA
| Variant (chain) | EDIII-DENV-1
|
EDIII-DENV-2
|
EDIII-DENV-3
|
EDIII-DENV-4
|
||||
|---|---|---|---|---|---|---|---|---|
| KD (nM) | Relative Affinity | KD (nM) | Relative Affinity | KD (nM) | Relative Affinity | KD (nM) | Relative Affinity | |
| 4E5A | 0.27 | 0.10 | 15.8 | 94.08 | ||||
|
| ||||||||
| T33V (HC) | <0.1 | >2.7 | <0.1 | >1.0 | 0.7 | 22.6 | 3.45 | 27.3 |
|
| ||||||||
| K31Q, T33V | <0.1 | >2.7 | <0.1 | >1.0 | 1.1 | 14.4 | 4.13 | 22.8 |
|
| ||||||||
| G27Y, F28W | <0.1 | >2.7 | <0.1 | >1.0 | 14.5 | 1.1 | 5.35 | 17.6 |
|
| ||||||||
| A25Δ (HC) | 1.27 | 0.21 | <0.3 | >0.33 | 19.9 | 0.79 | 297.00 | 0.32 |
|
| ||||||||
| S26Δ (HC) | <0.3 | >0.90 | <0.3 | >0.33 | 5.5 | 2.87 | 13.80 | 6.82 |
|
| ||||||||
| G27Δ (HC) | <0.3 | >0.90 | <0.3 | >0.33 | 9.6 | 1.65 | 25.76 | 3.65 |
|
| ||||||||
| G27P (HC) | 4.58 | 0.06 | <0.3 | >0.33 | 37.4 | 0.42 | 1936.00 | 0.05 |
|
| ||||||||
| G27A (HC) | <0.3 | >0.90 | <0.3 | >0.33 | 22.3 | 0.71 | 44.70 | 2.10 |
|
| ||||||||
| Y106R (HC) | <0.3 | >0.90 | <0.3 | >0.33 | 31.3 | 0.50 | 318.50 | 0.30 |
|
| ||||||||
| E97R (LC) | 0.36 | 0.75 | <0.3 | >0.33 | 31.6 | 0.50 | 120.55 | 0.78 |
|
| ||||||||
| E97K (LC) | 0.37 | 0.73 | <0.3 | >0.33 | 29.1 | 0.54 | 112.20 | 0.84 |
|
| ||||||||
| E97Q (LC) | 0.45 | 0.60 | <0.3 | >0.33 | 26.1 | 0.61 | 123.25 | 0.76 |
|
| ||||||||
| Humanized 4E5A | <0.1 | >2.70 | <0.1 | >1.0 | 5.8 | 2.72 | 55.08 | 1.71 |
|
| ||||||||
| Ab513 | <0.1 | >2.7 | <0.1 | >1.0 | 1.2 | 13.2 | 4.32 | 21.8 |